Molecular Biology Services
(Please read below, service terms included)
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Service Type
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Price (CNY)
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Description
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190
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Regular plasmid DNA Midiprep. One midiprep is enough for 3 "Service Quantity" | |
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320
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BAC Midiprep (<30 kb) plasmid DNA isolation | |
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450
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BAC Maxiprep (30-80 kb) plasmid DNA isolation | |
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570
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BAC (P[acman]) Maxiprep (>80 kb) plasmid DNA isolation | |
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740@
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Transposable element mapping for Plan B/C transformants. The final charge will be added when the number of transformant is reported | |
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740@
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Molecular mapping for Plan RH/RI CRISPR HDR transformants. The final charge will be added when the number of CRISPR HDR transformant is reported | |
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240+80@
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Genomic PCR confirmation of insertion on landing sites for Plan H or Plan I. The final charge will be added when the number of transformants is reported | |
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320+160@
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Genomic PCR confirmation of insertion on landing sites for PhiC31-RMCE. The final charge will be added when the number of transformants is reported。 | |
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640+480@
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For Plan M only. PCR confirmation of the attP sites for the new MiMIC strain, attB construct and construct/strain background. The ￥640 charge includes 1 strain and 1 construct, additional construct for the same strain costs ￥480 per construct | |
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320+160@
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Orientation screening for Plan M lines. Must order "Service W3" if you order "Service W4". The final charge will be added when the number of transformant is reported (see below detailed Service W4 descriptions) |
Service Z — DNA preparation Services: We also provide DNA preparation assistance. Because the fresh made DNA with high degree of supercoiled form and without freeze-thaw cycle is beneficial for the transformation efficiency, DNA prep by us is preferrable.
Service Z1 — Regular Midiprep (CNY190): One prep is good for 3x service quantity.
Service Z2 — BAC Midiprep for construct smaller than 30 kb (CNY320): For P[acman] construct require the EPI300 cell and induction before the DNA prep.
Service Z3 — BAC Maxiprep for construct between 30-80 kb (CNY450): For P[acman] construct require the EPI300 cells and induction before the DNA prep.
Service Z4 — BAC Maxiprep for construct larger than 80 kb (CNY570): For P[acman] constructs require the EPI300 cell and induction before the DNA prep.
We highly recommend you to include the proper plasmid map and/or sequence along with your sample so that we can do a quality control digest after the DNA prep and email the report to you (free of charge). If the digestion results are inconsistent,we will discuss with you the possible reason and try to fix the problem.
Service X1 - Transposable element mapping for Plan B/C transformants (CNY740 per line): When you order "Plan B+X1" or "Plan C+X1", we will make genomic DNA extracts of the transposable element transforming flies to be digested with proper restriction enzyme, followed by ligation and PCR reactions with primers appropriate for the ends of the transposable element.
We will test THREE lines per construct by default (you can request to test more, please let us know). Because we cannot predict how many lines will be transformed, we will request the payment after the service. The service report, containing the PCR fragment sequences and the cytosites, will be sent to you by email.
When you order Plan E or Plan F and need mapping for the transformants, please email us to discuss the possible services.
Service X2 - Molecular mapping for Plan RH/RI CRISPR HDR transformants (CNY740 per line): When you order "Plan RH+X2" or "Plan RI+X2", we may make genomic DNA extracts of CRISPR HDR transforming flies, perform PCR reactions with primers appropriate for both end of the HDR insertion.
We will test TWO lines per CRISPR injection by default (you can request to test more, please let us know). Because we cannot predict how many lines may be transformed, we will request the payment after the service. The service report, containing the PCR fragment sequences and characterizations, will be sent to you by email.
Service W1 - PCR confirmation of insertion on landing sites for Plan H or Plan I transformants (（CNY240 + CNY80 per additional line）per site): When you order "Plan H+W1" or "Plan I+W1", we will make genomic DNA extracts of the transformants flies for the PCR tests and send you the electrophoresis results.
We will test TWO lines per service by default (you can request to test more, let us know). Because we cannot predict how many lines will be transformed, we will request the payment after the service. For example, if you order 1 "Plan H+W1" service for gene-A into the 24483 strain, 1 "Plan I+W1" service for gene-B into the 24487 strain and 1 "Plan I+W1" service for gene-C into the 24488 strain and finally we got 5 w+ flies for each service, we will then only perform PCR for the first 2 w+ lines per service, i.e. (￥240+￥80)+(￥240+￥80)+(￥240+￥80)=￥960 for 3 sites transformants. The PCR results will be emailed to you after the shipment.
Service W2 - PCR confirmation for PhiC31-RMCE transformants ((CNY320 + CNY160 per additional lines) per site): When you order "Service W2”, we will make genomic DNA extracts of the transformant flies for the PCR tests and send you the electrophoresis results.
We will test TWO lines per construct by default (you can request to test more, please let us know). Because we cannot predict how many lines will be transformed, we will request the payment after the service.
Service W3 - for Plan M, PCR confirmation of the attP sites for the new MiMIC strain, construct and construct/strain background (CNY640 includes 1 strain and 1 construct, CNY480 per addition construct the same strain). We design the primers, order the oligos, perform genomic DNA extractions and perform the PCR works for you. This is very important to verify the strain and the construct before/for the long Plan M progress.
Service W4 - Orientation screening for Plan M lines (CNY320 + CNY160 per additional lines). Must order "Service W3" if you order "Service W4". By default, we only test 4 lines if we get 4 or more lines. i.e. the minimum charge is ￥400 if we only get one line. e.g. If we get 8 lines we only test the 4 lines, i.e. ￥320+￥160+￥160+￥160=￥800. If you want to test more, please email us.
